Mature cartilage marker acan was not changed

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We considered two possible reasons for lower specific activity of PKR in nontransformed cell lines: (1) the carcinoma cell lines may contain an activator of PKR, and (2) the nontransformed cell lines may contain an inhibitor of PKR. To investigate these possibilities, we performed a mixing experiment. The lysates of carcinoma cells and the nontransformed cells were mixed and preincubated for 10 min before PKR activity was assayed.

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